Journal: Cell Death & Disease

Article Title: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment

doi: 10.1038/cddis.2013.88

Figure Lengend Snippet: Characterization of HS-181 cell line after treatment with zebularine. ( a ) RT-PCR of cardiac markers after treatment with zebularine. Myh7, Myh6, Actc, cTnI and Serca2 present more expression after treatment. ( b ) Immunostaining of treated cells to detect cardiac-specific proteins. Scale bars; 50 μ m. ( c ) Blots of Proteome Profiler Array and the resulting quantification histograms demonstrating inhibition of pluripotency marker expression and ( d ) increased levels of mesodermic proteins after zebularine treatment (black arrows)

Article Snippet: Protein expression profiles were assayed using the specific human pluripotent Stem Cell array kit ‘Proteome Profiler Array' (R&D Systems Europe, Abingdon, UK; ARY010) following the manufacturer's instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Inhibition, Marker

Journal: Cell Stress & Chaperones

Article Title: Hypoxia- and mechanical stress–induced upregulation of mitochondrial HSP60 is associated with phenotypic switching of pulmonary arterial smooth muscle cells

doi: 10.1016/j.cstres.2025.100089

Figure Lengend Snippet: Inflammatory cytokine levels in supernatant of PASMCs cultured under different conditions . Detection of inflammatory cytokines was performed by Proteome Profiler™ Human Cytokine Array. Representative array images (left) and semiquantitative data (right) are shown.

Article Snippet: Human cell stress proteins were subsequently detected using the Proteome Profiler Human Cell Stress Array Kit (#ARY018, R&D Systems, Minneapolis, USA), following the manufacturer's instructions.

Techniques: Cell Culture

Journal: Nature

Article Title: High frequency of shared clonotypes in human B cell receptor repertoires

doi: 10.1038/s41586-019-0934-8

Figure Lengend Snippet: Summary of sequencing methods and cell counts

Article Snippet: , AbHelix® Human B and T Cell Profiling Assay , Full-length , 9 × 10 8 , 4.3 × 10 7 , 7.2 × 10 7 , HiSeq PE-250 , AbHelix, LLC.

Techniques: Sequencing

Journal: International Journal of Clinical Practice

Article Title: Machine Learning-Based Integrated Analysis of PANoptosis Patterns in Acute Myeloid Leukemia Reveals a Signature Predicting Survival and Immunotherapy

doi: 10.1155/2024/5113990

Figure Lengend Snippet: Identification of the diverse patterns based on the PANoptosis gene list. (a) A total of 226 PANoptosis-related genes belonging to necroptosis, apoptosis, and pyroptosis were collected for downstream analysis. (b) The univariate Cox regression analysis and Spearman's sum rank test were performed in the HOVON cohort, which was set as the training cohort. 28 genes with prognostic prediction were screened out with p value <0.05. (c) An interaction network of prognostic PANoptosis-related genes. (d) The 618 patients in HOVON cohort were divided into three clusters, according to the consensus clustering analysis based on the transcriptome profile of PANoptosis-related genes. (e) The KM curves showing the differential overall survival among the three subgroups. (f) A heat map showing the expression of the prognostic PANoptosis related genes in different subgroups. The two-sided p value <0.05 was considered significant for all statistical analyses and shown as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: Transcriptome data of human cancer cell lines (CCLs) were downloaded from the Broad Institute-Cancer Cell Line Encyclopedia project (CCLE, https://sites.broadinstitute.org/ccle/ ) and the CERES scores, the score to evaluate the dependency of the certain gene in the CCL, which indicated that the score was negative correlation with the possible significance of the gene in cell proliferation of the certain CCL, were downloaded from the dependency map portal (DepMap, https://depmap.org/portal/ ).

Techniques: Expressing